> Range of assays > Genomic stability: Karyotyping assays > iCS-digital™ PSC > iCS-digital™ PSC kit > iCS-digital™ PSC kit – DNA controls
Have you ever been in a situation where the results obtained from your digital PCR experiment did not make sense and you wished you had proper reference standards in place?
To help you regain control over your experiments and increase your quality standards, Stem Genomics has developed a range of ready-to-use positive controls. They are designed to help you understand any variabilities and identify potential errors in your workflow.
This panel of human controls can be used with our iCS-digital™ PSC kit range. It includes abnormalities found on all the targeted positions in our 24-probe or 20q kits, ie. the most frequent abnormalities found in human pluripotent stem cells. (ref. Assou 2020)
These controls enable you to:
Match the control with the corresponding mix. For example, Control 1 must be analyzed with MIX 1, Control 2 with MIX 2, etc …
Product
Quantity (volume)
Content
Control 1
20 reactions (60 µL – 5 ng/µL)
Positive control DNA (male) with Xp, 12p and 20q anomalies.
Control 2
20 reactions (60 µL – 5 ng/µL)
Positive control DNA (male) with 18q, 9q and 17q anomalies.
Control 3
20 reactions (60 µL – 5 ng/µL)
Positive control DNA (male) with 1q, 17p and 5q anomalies.
Control 4
20 reactions (60 µL – 5 ng/µL)
Positive control DNA (male) with 11p, 13q and 7q anomalies.
Control 5
20 reactions (60 µL – 5 ng/µL)
Positive control DNA (male) with 1p, 4q and 3p anomalies.
Control 6
20 reactions (60 µL – 5 ng/µL)
Positive control DNA (male) with 19p, 14q and 8q anomalies.
Control 7
20 reactions (60 µL – 5 ng/µL)
Positive control DNA (male) with 15q, 6q and 7p anomalies.
Control 8
20 reactions (60 µL – 5 ng/µL)
Positive control DNA (male) with 22q, 16q and 2q anomalies.
You can choose between using the full panel of controls (all the controls from 1 to 8), or buying them “à la carte”.
Product
Quantity (volume)
Content
Control 20q-only
20 reactions (60 µL – 5 ng/µL)
Positive control DNA (male) with 20q anomaly.
Product
Quantity (volume)
Content
Normal control
10 tests (240 µL – 5 ng/µL)
Normal control DNA (male) with CNV=1 at the ChrXp region and CNV=2 in the other 23 regions.
Control 1 to 8
Each consisting of a positive control DNA (male) with a set of 3 anomalies (CNVs).
20q control
Normal control DNA (XY)
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