iCS-digital™ PSC:
fast in-routine genomic testing assay

Detect over 93% of recurrent abnormalities
in hPSCs in record-breaking time!

Human Pluripotent Stem Cells (hPSCs) are particularly prone to developing abnormalities during their time in culture. In 5 passages or less, the variants that appear can rapidly become predominant, compromising your research work.

Traditional G-Banding karyotyping is helpful in providing an exhaustive structural and numerical variant analysis. However, it does not have the required sensitivity to identify the most frequent genomic defects in human pluripotent stem cells.

Additionally, hPSC research scientists need assays designed for in-process testing every 5-10 passages (Assou et al., 2020; McIntire et al., 2020; Pamies et al., 2017) in order to pick up abnormalities as early as possible in their workflow, and G-Banding is not designed to support that level of frequency in testing.

The sensitivity and speed required in delivering a reliable assessment of genomic stability in hPSCs led Stem Genomics to develop the iCS-digital™ PSC range.

High level of performance for optimum detection

Thanks to the high level of performance offered by digital PCR (200 bp and 20% mosaicism) combined with an in-depth analysis of most recurrent abnormalities in hPSCs (see SMART database), the iCS-digital™ PSC range of tests can detect sub-karyotyping abnormalities that G-Banding would miss.

Our iCS-digital™ PSC 28-probe test (available as a service only) will capture over 93% of recurrent defects in hPSCs, including the 20q11.21 amplification that accounts for 1⁄4 of recurrent abnormalities in hPSCs worldwide.
Our iCS-digital™ PSC 24-probe test (available as a kit only) will capture over 90% of recurrent defects in hPSCs, including the 20q11.21 amplification.
Our iCS-digital™ PSC 20q-only test focuses on the gain of 20q11.21 copy-number variant (CNV). It is detected in more than 20% of worldwide cultured human Pluripotent Stem Cells (hPSCs) and represents 22.9% of the recurrent structural variants identified in hPSCs (Assou et al., 2020)[1], making it the most common genomic abnormality in hPSCs.3

iCS-digital™ PSC key specifications at a glance

Cell types

Human PSCs:
ESCs & iPSCs

Stages

In-process control during cell amplification & maintenance

Clone screening

Banking characterization

Samples

gDNA

Cell pellet

Shipment

Room temperature

Dry ice

Coverage

Test with 28 probes: 93% of recurrent abnormalities

Test with 24 probes: 90% of recurrent abnormalities

Test the 20q11.21 region: the most common genomic abnormality in hPSCs (>20%)

Time

2-3 days after sample reception

Available as a service
(from one sample) or a kit:

For research use only

Practical testing guidelines

Recommended guidelines in terms of genomic stability advise checking the cells every 5-10 passages during their time in culture, but also screening clones after any stressing process (reprogramming, gene editing, etc.) and during banking. It is also advisable when a new line is acquired.

Click on the graph for more details:

Successful differentiation starts with high quality material

If your raw material is not stable, it is difficult to trust any differentiation changes down the line. Therefore, whilst we are verifying your cell line pluripotency, why not also check for potential genomic abnormalities or mycoplasma at the same time? We have fast and high-performing digital PCR tests that will enable you to perform these tests simultaneously. This will also enable you to benefit from additional discounts.

New: you can now order up to 5 tests designed specifically for pluripotent stem cell workflows in one go.

Watch a quick video presentation of the one-stop-shop service

Play

It’s up to you to...

SMART database

In order to accurately target the abnormalities relevant for hPSC researchers, Stem Genomics specifically analysed the data from 132 scientific publications concerning 1485 hPSC samples. After exclusion of polymorphic variants, we highlighted the presence of 949 recurrent genetic abnormalities (i.e., genomic defects found in at least five different publications).

We then used these data to develop the iCS-digital™ PSC test.

The test is published in Stem Cell Reports (Assou et al., 2020).

See what our customers say

EditCo Bio

| Alexander Brown, Associate director, Scientific Development and Montse Morell, Director of scientific development.

“We evaluated a lot of karyotyping technologies and only Stem Genomics’ digital PCR-based assay enabled the scalability, sensitivity, repeatability, and speed we needed to rapidly iterate and improve our processes. In addition, we have automated much of our cell-editing workflow and we found that only this technology was compatible with the speed and scale that we wanted in-house”.

Ocular Genomics Institute, Harvard Medical School

| Dr Marcela Garita-Hernandez, Pharm D, PhD, Director of the iPSC Research Program, Pierce Lab.

“In my experience, you need to check your cell lines routinely, as nothing that I have found to date can help you predict genomic abnormalities without testing your cell lines regularly. If your raw material is not stable, it is very difficult to trust any differentiation changes down the line. iCS-digital™ PSC is ideal for regular testing and has always been part of our workflow. It is also affordable for most labs, so there is really no excuse not to test your lines”.

Ncardia

| Arie Reijerkerk, Director Manufacturing Technology

The results are obtained very quickly; the reports are clearly interpreted and there is a smooth line of communication between Ncardia and Stem Genomics whenever we require further clarifications.

University of Freiburg Department of Cardiology and Angiology, AG Hilgendorf

| Dr. Tsai-sang Dederichs, scientist

With Duo iCS-Karyo, Stem Genomics provides a very convenient and good quality service that makes genomic quality control easy. Communication is smooth and instructions are clear.

Cellected

| Claire Richards, CEO and Founder

I would recommend the iCS-digital™ PSC to anyone who wants a good way of keeping an eye on their cells almost in real time and as a complement to traditional techniques that take a lot longer.

DiNAQOR

| Kurt Jacobs, Research scientist

The iCS-digital™ PSC 24-probe kit covers a wide range of mutations in a simple test. It helps us strengthen our quality control at various stages of our workflow. For instance, it picked up the 20q amplicon that was present in some of our hiPSC lines.

denovoMATRIX

| Sandra Segeletz, Head of Innovation

I would recommend the iCS-digital™ tests for anyone looking for a good genomic appraisal at minimum effort. In addition, this is an easy entry to quality control for anyone with a limited budget.

GoLiver Therapeutics

| Angélique Fourrier, R&D project manager

Since we started using the iCS-digital™ PSC for routine control, we have gained a massive degree of confidence in the quality of our cells in long-term culture […] Not surprisingly, by removing the genetic instability factor from the equation, we are rewarded with a highly scalable, efficient and very cost-effective GMP differentiation process for PSCs to produce safe live cell therapy products on a multi-billion cell scale.

ICM

| Stéphanie Bigou, Responsable Opérationnelle

We have been using the (iCS-Digital™) tests since their launch in 2018 and find them to be very reliable.

Published scientific articles
citing the iCS-digital™ PSC assay

2024

Generation of three iPSC lines with inducible systems to be used in Angelman syndrome research

Josephine Haake a , Maren Kaufmann a , Laura Steenpass a,b,*

Link to the publication

2024

hsa-miRNA-548v controls the viscoelastic properties of human cardiomyocytes and improves their relaxation rates

Eva Vermersch1,2 PhD, Salomé Neuvendel1, Charlene Jouve1, Andrea Ruiz Velasco1, Céline Pereira1, Magali Seguret1, Marie-Elodie Cattin-Messaoudi2 PhD, Sofia Lotfi2, Thierry Dorval2 PhD, Pascal Berson3 PhD, and Jean-Sébastien Hulot1,4* MD PhD.

Link to the publication

2023

Lab Resource: Single Cell Line Generation of an induced pluripotent stem cell line (ITXi012-A) from a patient with genetically determined high-lipoprotein(a) plasma levels

Amandine Caillaud a, Lise Bray a, Aurore Girardeau a, Zoé Begué-Racapé a, Lucie Vince a, Murielle Patitucci a, Cédric Le May a, Gilles Lambert b, Bertrand Cariou a, Antoine Rimbert a

Link to the publication

2023

Establishment of induced pluripotent stem cells IRMBi005-A from a patient with sporadic Alzheimer’s disease

C. Clua Provost a 1, L. Auboyer b 1, A. Rovelet-Lecrux c, C. Monzo a, Eliot Schob a, S. Lehmann a, D. Wallon d, C. Crozet a b

Link to the publication

2023

Lab Resource: Multiple Cell Lines Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative

Valentina Balducci a 1, Francesco Scardigli b 1, Magdalena Harakalova b c, J. Peter. van Tintelen d, Pieter A. Doevendans b e, Kevin D. Costa f, Irene C. Turnbull f, Joost P. G. Sluijter b c, Francesca Stillitano b c f

Link to the publication

2023

Lab Resource: Genetically-Modified Multiple Cell Lines Generation of gene corrected human isogenic iPSC lines (IDVi003-A_CR13, IDVi003-A_CR21, IDVi003-A_CR24) from an inherited retinal dystrophy patient-derived IPSC line ITM2B-5286-3 (IDVi003-A) carrying the ITM2B c.782A>C variant using CRISPR/Cas9

Tasnim Ben Yacoub1, Camille Letellier1, Juliette Wohlschelegel1, Christel Condroyer1, Amélie Slembrouck-Brec1, Olivier Goureau1, Christina Zeitz1, Isabelle Audo1,2,3

Link to the publication

2023

AAV-mediated gene augmentation therapy of CRB1 patient-derived retinal organoids restores the histological and transcriptional retinal phenotype

Nanda Boon, Xuefei Lu, Charlotte A. Andriessen, Ioannis Moustakas, Thilo M. Buck, Christian Freund, Christiaan H. Arendzen, Stefan Bo¨hringer, Hailiang Mei, and Jan Wijnholds.

Link to the publication

2023

Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads

Albert Blanch-Asensio, Babet van der Vaart, Mariana Vinagre, Eline Groen, Christiaan Arendzen, Christian Freund, Niels Geijsen, Christine L. Mummery, Richard P. Davis.

Link to the publication

2022

Modeling PRPF31 retinitis pigmentosa using retinal pigment epithelium and organoids combined with gene augmentation rescue.

Rodrigues A, Slembrouck-Brec A, Nanteau C, Terray A, Tymoshenko Y, Zagar Y, Reichman S, Xi Z, Sahel JA, Fouquet S, Orieux G, Nandrot EF, Byrne LC, Audo I, Roger JE, Goureau O. Modeling PRPF31 retinitis pigmentosa using retinal pigment epithelium and organoids combined with gene augmentation rescue. NPJ Regen Med. 2022 Aug 16;7(1):39. doi: 10.1038/s41536-022- 00235-6. PMID: 35974011; PMCID: PMC9381579.

Link to the publication

2022

CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles.

Mianné J, Nasri A, Van CN, Bourguignon C, Fieldès M, Ahmed E, Duthoit C, Martin N, Parrinello H, Louis A, Iché A, Gayon R, Samain F, Lamouroux L, Bouillé P, Bourdin A, Assou S, De Vos J. CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles. BMC Biol. 2022 Jan 7;20(1):8.

Link to the publication

2021

PCSK9 regulates the NODAL signaling pathway and cellular proliferation in hiPSCs.

Roudaut M, Idriss S, Caillaud A, Girardeau A, Rimbert A, Champon B, David A, Lévêque A, Arnaud L, Pichelin M, Prieur X, Prat A, Seidah NG, Zibara K, Le May C, Cariou B, Si-Tayeb K. PCSK9 regulates the NODAL signaling pathway and cellular proliferation in hiPSCs. Stem Cell Reports. 2021 Dec 14;16(12):2958-2972.

Link to the publication

2021

Optogenetically controlled human functional motor endplate for testing botulinum neurotoxins.

de Lamotte JD, Polentes J, Roussange F, Lesueur L, Feurgard P, Perrier A, Nicoleau C, Martinat C. Optogenetically controlled human functional motor endplate for testing botulinum neurotoxins. Stem Cell Res Ther. 2021 Dec 5;12(1):599.

Link to the publication

iCS-digital™ PSC:
fast in-routine genomic testing assay

Detect over 93% of recurrent abnormalities
in hPSCs in record-breaking time!

High level of performance for optimum detection

iCS-digital™ PSC key specifications at a glance

Practical testing guidelines

Successful differentiation starts with high quality material

It’s up to you to...

SMART database

See what our customers say

Published scientific articles
citing the iCS-digital™ PSC assay

Generation of three iPSC lines with inducible systems to be used in Angelman syndrome research

hsa-miRNA-548v controls the viscoelastic properties of human cardiomyocytes and improves their relaxation rates

Lab Resource: Single Cell Line Generation of an induced pluripotent stem cell line (ITXi012-A) from a patient with genetically determined high-lipoprotein(a) plasma levels

Establishment of induced pluripotent stem cells IRMBi005-A from a patient with sporadic Alzheimer’s disease

Lab Resource: Multiple Cell Lines Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative

Lab Resource: Genetically-Modified Multiple Cell Lines Generation of gene corrected human isogenic iPSC lines (IDVi003-A_CR13, IDVi003-A_CR21, IDVi003-A_CR24) from an inherited retinal dystrophy patient-derived IPSC line ITM2B-5286-3 (IDVi003-A) carrying the ITM2B c.782A>C variant using CRISPR/Cas9

AAV-mediated gene augmentation therapy of CRB1 patient-derived retinal organoids restores the histological and transcriptional retinal phenotype

Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads

Modeling PRPF31 retinitis pigmentosa using retinal pigment epithelium and organoids combined with gene augmentation rescue.

CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles.

PCSK9 regulates the NODAL signaling pathway and cellular proliferation in hiPSCs.

Optogenetically controlled human functional motor endplate for testing botulinum neurotoxins.

Download this file immediately

Access the webinar immediately

Upcoming webinar

Upcoming webinar

Download this file immediately

iCS-digital™ PSC: fast in-routine genomic testing assay

Detect over 93% of recurrent abnormalities in hPSCs in record-breaking time!

High level of performance for optimum detection

iCS-digital™ PSC key specifications at a glance

Practical testing guidelines

Successful differentiation starts with high quality material

It’s up to you to...

SMART database

See what our customers say

Published scientific articles citing the iCS-digital™ PSC assay

Generation of three iPSC lines with inducible systems to be used in Angelman syndrome research

hsa-miRNA-548v controls the viscoelastic properties of human cardiomyocytes and improves their relaxation rates

Lab Resource: Single Cell Line Generation of an induced pluripotent stem cell line (ITXi012-A) from a patient with genetically determined high-lipoprotein(a) plasma levels

Establishment of induced pluripotent stem cells IRMBi005-A from a patient with sporadic Alzheimer’s disease

Lab Resource: Multiple Cell Lines Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative

Lab Resource: Genetically-Modified Multiple Cell Lines Generation of gene corrected human isogenic iPSC lines (IDVi003-A_CR13, IDVi003-A_CR21, IDVi003-A_CR24) from an inherited retinal dystrophy patient-derived IPSC line ITM2B-5286-3 (IDVi003-A) carrying the ITM2B c.782A>C variant using CRISPR/Cas9

AAV-mediated gene augmentation therapy of CRB1 patient-derived retinal organoids restores the histological and transcriptional retinal phenotype

Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads

Modeling PRPF31 retinitis pigmentosa using retinal pigment epithelium and organoids combined with gene augmentation rescue.

CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles.

PCSK9 regulates the NODAL signaling pathway and cellular proliferation in hiPSCs.

Optogenetically controlled human functional motor endplate for testing botulinum neurotoxins.

Download this file immediately

Access the webinar immediately

Upcoming webinar

Upcoming webinar

Download this file immediately

iCS-digital™ PSC:
fast in-routine genomic testing assay

Detect over 93% of recurrent abnormalities
in hPSCs in record-breaking time!

Published scientific articles
citing the iCS-digital™ PSC assay