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Genomic abnormalities can lead to the potential development of tumors in patients and reduce the efficacy of MSC treatments (Neri, 2019). Traditional G-Banding karyotyping is helpful in providing an exhaustive structural and numerical variant analysis. However, it can be long and difficult to organize. This led Stem Genomics to develop a specific solution for research scientists working on hMSCs.
iCS-digital™ MSC has been designed to capture 80% of abnormalities found in MSCs.
We carried out an exhaustive meta-analysis of genomic abnormalities and referenced about 300 genetic abnormalities in hMSC lines. We then used this data to design our own assay: a set of 24 probes optimized to target all human chromosomes.
Our digital PCR-based solution enables rapid and cost-effective screening for chromosomal abnormalities, delivering results in just 3 days.
iCS-digital™ MSC can also complement a process already in place and bring an extra layer of precision to your QC.
Human MSCs
After isolation
During cell expansion
Before banking
gDNA
Cell pellets
Cells in fresh culture media (or in cell culture supernatant)
Room temperature
Dry ice
Room temperature
>80% of recurrent abnormalities
>20% (depending on sample quality)
2-3 days after sample reception
Recommended guidelines in terms of genomic stability advise checking the cells during their time in culture but also screening clones after any stressing process (reprogramming, gene editing, etc.) and during banking. It is also advisable when a new line is acquired.
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IRMB – Hôpital Saint Eloi
80 avenue Augustin Fliche
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