iCS-digital™ PSC kit:
your results in less than 48 hours!

Choose the convenience of Stem Genomics kits to perform genomic stability tests in-house

The iCS-digital™ PSC kit range is compatible with Bio-Rad QX ONE, QX100, QX200 and QX 600 machines.

Main benefits:

  • Available as 24 probes (92% of recurrent genomic abnormalities in hPSCs) or a 20q-only kit (the most recurrent chromosomal alteration found in hPSCs)
  • Easy to use
  • High reproducibility
  • Results in less than 48 hours
  • Automated analysis and interpretation

See what our customers say

EditCo Bio
| Alexander Brown, Associate director, Scientific Development and Montse Morell, Director of scientific development.
“We evaluated a lot of karyotyping technologies and only Stem Genomics’ digital PCR-based assay enabled the scalability, sensitivity, repeatability, and speed we needed to rapidly iterate and improve our processes. In addition, we have automated much of our cell-editing workflow and we found that only this technology was compatible with the speed and scale that we wanted in-house”.  
,,
DiNAQOR
| Kurt Jacobs, Research scientist
The iCS-digital™ PSC 24-probe kit covers a wide range of mutations in a simple test. It helps us strengthen our quality control at various stages of our workflow. For instance, it picked up the 20q amplicon that was present in some of our hiPSC lines.
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iCS-digital™ PSC kit specifications at a glance

24 probes

Species

Human

Cell types

hPSCs:
ESCs & iPSCs

Compatible instruments

QX ONE, QX100, QX200 and QX 600

Droplet Digital PCR

Bio-Rad system

Storage

-20°C
upon reception

Size

20 or 40 reactions

Coverage

91% of recurrent abnormalities

Mosaicism

> 20%
(depending on sample quality)

20q only

Species

Human

Cell types

hPSCs:
ESCs & iPSCs

Compatible instruments

QX ONE, QX100, QX200 and QX 600

Droplet Digital PCR

Bio-Rad system

Storage

-20°C
upon reception

Size

20 or 40 reactions

Coverage

The 20q11.21 gain is responsible for >20% of recurrent abnormalities

Mosaicism

> 20%
(depending on sample quality)

Workflow for one cartridge

iCS-digital™ PSC kit descriptions

24 probes

Additional reagents required, but not included

ddPCR™ Supermix for Probes (No dUTP)
– Bio-Rad (#186023)

HindIII-HF Enzyme
– e.g. New England Biolabs (#R3104L)

Kit documentation

20q only

iCS-digital™ 20q-only kit content

Mix1 to 8, each consisting of:
– 3 specific probe assays
– 1 reference probe assay

Normal control DNA (XY)

Additional reagents required, but not included

ddPCR™ Supermix for Probes (No dUTP)
– Bio-Rad (#186023)

HindIII-HF Enzyme
– e.g. New England Biolabs (#R3104L)

Kit documentation

Straightforward reporting directly accessible from your device

Once you have completed your experiment, simply upload the raw data to the web-based iCS-digital™ analysis software included in the kit.

The software will process the data and automatically generate the final interpreted report. Straightforward and clear!

Watch the tutorial for the iCS-digital™ software:
View useful documents

Upcoming webinar

Join us LIVE for our webinar “Genomic stability made easy” to discover how Stem Genomics has turned cutting-edge technologies into easy testing solutions accessible to most labs.

Get expert opinions and user perspectives on our genomic stability assays in the context of iPSC research.

Happening on Tuesday, November 14, 6 pm CET

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