It is best not to have too sparse or too dense a culture. If the cells are seeded too sparsely, it will not work. They need to have near neighbors, so if they are too thin in the culture, they do not do as well and tend to die out during shipping. At the other extreme, if the flask is nearly completely full, this will cause “contact inhibition”, slowing the growth level and causing them to start to die out as well. The aim is to achieve 40-60% confluency in the flask upon arrival. One characteristic of very healthy stem cells is that they will continue to grow during shipping outside the incubator. Also, even if the cells are in a polystyrene box, they will respond to outside temperature and warm conditions. Finally, Stem Genomics recommends using the service of a reliable overnight courier to keep the transportation time to a minimum, as it is a stressful stage for the cells. All this needs to be taken into consideration when sending your cells to us for karyotyping analysis.
Reviewers require at least a G-banding karyotype for checking genomic stability. The Duo iCS-Karyo test by Stem Genomics is accepted and will provide even more information than a single G-band analysis.
Not at all. It is a very straightforward report that gives you a clear indication of the abnormalities found. A sample report is available on request.
The price will depend on the number of samples you need to test, and you can benefit from volume-based discounts.
This is only possible with our US laboratory for now. Please enquire for the latest update in Europe.
In accordance with the ISSCR’s latest quality standard recommendations, Stem Genomics strongly recommends regularly checking the identity of your stem cells during their time in culture with our STR assay. Mycoplasma testing is also critical for robust science and we have a digital PCR solution called Myco-digital that can do that for you.
As of July 17, our new address will be:
400 Park Offices Drive, Room 105
Research Triangle Park, Durham, North Carolina 27713
Please note that we will be closed from July 12 to 16
during the transition.
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Join us LIVE for a Q&A session on the latest iCS-digital™ PSC upgrade.
Find out what it means for you and ask all your questions
Juline VINCENT our R&D Project Manager and Digital PCR expert will answer you LIVE.
SAVE THE DATE: Thursday, November 7, 6pm CET/9am PDT/12 pm EDT.
Join us LIVE for our webinar!
Best Practices: Making your hPSC Quality Control workflow more efficient
Join Juline VINCENT, our R&D Project Manager, as she presents efficient quality control strategies specifically designed to address the unique characteristics of pluripotent stem cells.
Thursday, October 23, 12:00 PM EST / 6:00 PM CET
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