Myco-digital PCR test: detect Mycoplasma contamination
The Myco-digital PCR test allows the fast and accurate detection of mycoplasma contamination in cell cultures.
Any cells
≥ 20 genome copies / PCR reaction
Cell culture supernatant (≥ 500 µL)
Room temperature
> 60 mycoplasma species, including the 6 species responsible for 90-95% of cell culture infections
2-3 days after sample reception
Digital PCR for the detection of mycoplasma contamination
Monitoring cell cultures for potential mycoplasma contaminations is mandatory to ensure the cell line safety and for high-quality research.
The Myco-digital test is a fast and accurate digital PCR-based test for mycoplasma detection in cell culture.
It has been precisely designed to target the conserved 16S ribosomal RNA coding region of the mycoplasma genome.
Based on sequence homology, the Myco-digital test can detect more than 60 species, including the six species responsible for about 90-95% of all cell culture mycoplasma infections (Acholeplasma laidlawii, Mycoplasma arginini, Mycoplasma fermentans, Mycoplasma hominis, Mycoplasma hyorhinis, and Mycoplasma orale).
Myco-digital results
A) Digital PCR 2D plots. Each dot represents one PCR reaction droplet. Top panel: positive control with a cluster of droplets with mycoplasma DNA (blue) and negative droplets (grey). Bottom panel: negative control with only negative droplets (grey).
B) Example of a Myco-digital results report.
As the Myco-digital test uses a supernatant sampling method, the test can be run alongside the iCS-digital TM tests for which cells-in-supernatant samples are being tested.
We provide a full testing service with a fast turnaround time (2-3 days after sample reception).
