Monitoring differentiation and identifying pluripotent stem cells are fundamental aspects of any pluripotent stem cell culture. Numerous publications in the scientific literature suggest genes and cell surface antigens that can be used for this purpose, along with a variety of available assays and methods. This can lead to confusion. With the Pluri-digital™ assay, Stem Genomics helps scientists monitor the undifferentiated markers cited by the ISSCR with a simple, cost-effective and rapid solution. This test provides valuable insight into the differentiation of a cell line, from its undifferentiated state to its trilineage differentiation profile in just 5 business days.
The Pluri-digital™ service will help you establish the pluripotency of your cell line rapidly within 5 business days. This cost-effective service has been designed for labs that choose to outsource and are seeking an affordable solution with a quick turn-around.
You can choose to check only the undifferentiated status of your cell line and/or their trilineage differentiation profile. If you’d like us to do both, two separate samples will be required.
Human cell lines: embryonic body differentiated cell lines, undifferentiated pluripotent cell lines and differentiated cell lines not coming from embryonic bodies.
RNA
Frozen cell pellets
Cells in RLT buffer
Undifferentiated genes: NANOG, DNMT3B, SOX2, POU5F1/OCT4
Ectoderm genes: MAP2, PAX6, EN1, NEFL
Mesoderm genes: HAND1, CDH5, MYOG, TBXT
Endoderm genes: AFP, FOXA2, SOX17, SST
Gene selection based on the ISSCR Standard recommendations and renowned scientific publications.
Dry ice
5 days after sample reception
If your raw material is not stable, it is difficult to trust any differentiation changes down the line. Therefore, whilst we are verifying your cell line pluripotency, why not also check for potential genomic abnormalities or mycoplasma at the same time? We have fast and high-performing digital PCR tests that will enable you to perform these tests simultaneously. This will also enable you to benefit from additional discounts.
New: you can now order up to 5 tests designed specifically for pluripotent stem cell workflows in one go. Watch a quick video presentation of the one-stop-shop service
Our experts specialized in stem cell characterization are here to help and recommend the procedure best adapted to your specific workflow.
The guidelines for checking the pluripotency of your cell lines recommend doing so after cloning at the reprogramming or gene editing stages in order to check the expression of undifferentiated markers. Pluri-digital™ can also be used during differentiation monitoring. Finally, we recommend using Pluri-digital™ to check the undifferentiated markers every time the cell line does not look or behave as expected.
Click on the graph for more details:
We are quick to respond
Let's talk to each other
According to your availability
Subscribe to stay informed
Cap Sigma
1682 rue de la Valsière
34 790 Grabels
5501 Fortunes Ridge Drive,
Suite O,
Durham, NC 27713
Please enter your details below
Please enter your details below
Join us LIVE for a Q&A session on the latest iCS-digital™ PSC upgrade.
Find out what it means for you and ask all your questions
Juline VINCENT our R&D Project Manager and Digital PCR expert will answer you LIVE.
SAVE THE DATE: Thursday, November 7, 6pm CET/9am PDT/12 pm EDT.
Join us LIVE for our webinar “Genomic stability made easy” to discover how Stem Genomics has turned cutting-edge technologies into easy testing solutions accessible to most labs.
Get expert opinions and user perspectives on our genomic stability assays in the context of iPSC research.
Happening on Tuesday, November 14, 6 pm CET
Please enter your e-mail address.
