Detect abnormalities in hPSCs: in-house kit
The iCS-digital TM PSC 24-probes kit allows the detection by digital PCR of more than 90% of recurrent
genomic abnormalities in human pluripotent stem cells (hPSCs).
DNA extraction & quantification
Digital PCR reagent preparation
Droplet generation
PCR
Droplet reader
Online analysis & interpretation
Human
hPSCs:
ESCs & iPSCs
QX100 and QX200
Droplet Digital PCR
Bio-Rad system
-20°C
upon reception
20 tests
91% of recurrent abnormalities
> 20%
(depending on sample quality)
The iCS-digital TM PSC kit is a precise and sensitive test for the detection of the most common genomic defects observed in cultured human Pluripotent Stem Cells (hPSCs). The kit, based on the digital PCR technology, allows the reliable quantification of 24 different DNA targets using eight multiplex assays (Mix 1 to 8). The kit also includes a validated normal genomic DNA control sample (XY).
Data processing, statistical analysis, and graphical representation of the results are performed in an automated and explicit way, thanks to the online iCS-digital TM analysis tool provided by Stem Genomics.
For Research Use Only
Example of test report generated using the iCS-digital TM software
for easy data analysis and interpretation.
The iCS-digital TM PSC kit allows the fast and easy in-house analysis of the most common
genomic defects occuring in hPSCs.
iCS-digital TM PSC kit content
Mix1 to 8, each consisting of :
- 3 specific probe assays
- 1 reference probe assay
Control DNA (XY)
Additional reagents required, but not included
ddPCR TM Supermix for Probes (No dUTP)
- Bio-Rad (#186023)
HindIII-HF Enzyme
- e.g. New England Biolabs (#R3104L)
