Detect the 20q amplicon in hPSCs: in-house kit
The iCS-digital TM PSC 20q-only kit allows the detection by digital PCR of the most recurrent
genomic defect in human pluripotent stem cells (hPSCs).
DNA extraction & quantification
ddPCR reagent preparation
Droplet generation
PCR
Droplet reader
Analysis
Human
hPSCs:
ESCs & iPSCs
QX100 and QX200
Droplet Digital PCR
Bio-Rad system
-20°C
upon reception
20 tests
The 20q11.21 gain is responsible for >20% of recurrent abnormalities
> 20%
(depending on sample quality)
The iCS-digital TM PSC 20q-only kit is based on the digital PCR (dPCR) technology and allows the reliable quantification of the sub-karyotypic 20q11.21 amplification. Gain of 20q11.21 copy-number variant (CNV) is detected in more than 20% of worldwide cultured human Pluripotent Stem Cells (hPSCs) 1-3 and represents 22.9% of the recurrent structural variants identified in hPSCs 4, making it the most common genomic abnormality in hPSCs.
Cells harbouring the 20q11.21 amplification display a selective advantage and can completely overtake the culture in few passages 5.
Detection of the 20q11.21 amplification using a dedicated technology is critical because most of these mutations fall below the size detection limit of conventional G-banding karyotyping (as illustrated on the right, panel B).
The kit also includes a validated normal genomic DNA control sample.
For Research Use Only
1, 2, 3, 4, 5 Refer to scientific publication number 1, 2, 3, 4 or 5 (bottom of page).
Sub-karyotypic 20q11.21 duplication in hPSCs (A) detected using the iCS-digital TM PSC 20q-only kit (sample 3) and (B) not identified by G-banding karyotyping method.
Images are for illustrative purposes only.
The iCS-digital TM 20q-only kit allows the fast and easy in-house analysis of the sub-karyotypic 20q11.21 amplification in hPSCs
iCS-digital TM 20q-only kit content
Mix 20q that consists of :
- 1 specific probe assay
- 1 reference probe assay
Control DNA (XY)
Additional reagents required, but not included
ddPCR TM Supermix for Probes (No dUTP)
- Bio-Rad (#186023)
HindIII-HF Enzyme
- e.g. New England Biolabs (#R3104L)